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David Eide

Professor of Nutritional Sciences

David Eide
Lab Home Page:
Eide Lab
Address:
340B Nutritional Sciences
Telephone:
263-1613
Email:
eide@nutrisci.wisc.edu
Research Fields:
Yeast and Fungi
Gene Expression
Genomics

Ph.D., University of Wisconsin-Madison (1987)

Postdoctoral Research: MIT (1987-1990). University of Utah (1990-1991)

Research Interests

Mechanisms and regulation of metal ion homeostasis in yeast and other eukaryotes

Research Description

Our research is focused on the mechanism of transition metal uptake in eukaryotic cells and how this process is regulated in response to changes in the availability of metal ions in the diet or the environment. We are using the yeast Saccharomyces cerevisiae as a model system for understanding these processes in mammals and plants.

Questions our research are currently addressing include the following:

  1. What transport proteins are responsible for the uptake of metals and their intracellular distribution in yeast and how do they work? We have identified metal uptake transporters in the plasma membrane and are currently searching for transporters involved in putting metals into mitochondria and the secretory pathway.
  2. How is the activity of these transporters regulated in response to metal-deprivation and excess? We have identified both transcriptional and post-transcriptional regulatory mechanisms and are studying how these systems work at the molecular level.
  3. Can we use the reagents generated in our yeast studies to identify metal transporters from higher eukaryotes? Absolutely! We have identified metal transporters in plants and mammals that are related to the yeast proteins and we are currently studying their function and regulation.

Representative Publications

  • Wu, C., Bird, A., Winge, D. and Eide, D. 2007. Regulation of the yeast Tsa1 peroxiredoxin by Zap1 is an adaptive response to the oxidative stress of zinc deficiency. J. Biol. Chem. 282:2184-2195.
  • Simm, C., Lahner, B., Salt, D., LeFurgey, A., Ingram, P., Yandell, B., and Eide, D. 2007. The yeast vacuole in zinc storage and intracellular zinc distribution. Eukaryotic Cell, 6:1166-1177.
  • Bird, A., Gordon, M., Eide, D., and Winge, D. 2006. Repression of ADH1 and ADH3 during zinc deficiency by Zap1-induced intergenic RNA transcripts. EMBO J. 25:5726-5734.
  • Qiao, W., Mooney, M., Bird, A., Winge, D., and Eide, D. 2006. Zinc binding to a regulatory zinc-sensing domain monitored in vivo using fluorescence resonance energy transfer. Proc. Natl. Acad. Sci. USA. 103:8674-8679.
  • Ellis, C., MacDiarmid, C., and Eide, D. 2005. Heteromeric protein complexes mediate zinc transport into the secretory pathway of eukaryotic cells. J. Biol. Chem. 280:28811-28818.